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Effect of Probiotics on the Growth and Food Utilization of Walking Catfish (Clarias batrachus) under Laboratory Condition of IAAS, Paklihawa
Shailesh Gurung*, Sumnima Dhakal1, Ashish Chaudhary1, Prabin Paudel1, Birendra Shrestha1, Dipendra Mishra1
1Tribhuvan University, Institute of Agriculture and Animal Science, Bhairahawa, Nepal
Abstract
The experiment was conducted in Aquaculture lab of IAAS, Paklihawa, Rupandehi district from 6th February 2018 to 6th April 2018. The research was conducted for the evaluation of the effect of different probiotics on growth and feed utilization of Catfish (Clarias batracus) under laboratory condition. One hundred and  sixty eight catfish fries with average initial weight 1.60-1.70gm  were stocked in 12 glass aquarium tank of size 12*24*12 each half-filled with water and aerated continuously using an air compressor. The stocking density of catfish fries was 14 fish per aquarium. The experiment was performed at completely randomized block design with 4 treatments and 3 replications. The treatments were T1 Control (Rice bran + MOC), T2 (Rice bran + MOC + Microguard), T3 (Rice bran + MOC + Nicoli) and T4 (Rice bran + MOC + Grozyme). Probiotics i.e. Microguard, Grozyme and Nicoli was each applied at the rate of 2 % of total weight of supplementary feed (Rice bran and MOC) in T2, T3 and T4 respectively. Feed was allocated to each aquaria at the rate of 8 % of total body weight of fish. Water quality parameters pH, DO and Temperature was measured on weekly basis and Ammonia, Nitrate and Nitrite was measured fortnightly. The average pH varied from 8.1 to 8.4 within 2 months while the DO varied from 5.4 mg/l to 7.9 mg/l. The temperature was found optimum ranging from 19.6oC to 24.10C during the experimental period. Mean nitrate concentration ranged from 0.1 to 2.67 ppm concentration while mean nitrite level ranged from 0ppm to 3.5ppm and the mean ammonia level range from 0.21 ppm to 5.33 ppm. The mean total harvest weight of T2 (Feed mixed with Probiotics/Microguard) was found highest with 39.66 gm seemed fairly high in comparison to the other treatments during the experimental period. Considerably, the mean individual harvest weight in T2 was also found highest with 2.99 gm among other treatments. Moreover, Specific Growth Rate (SGR) of catfish in T2 during the experimental period was also display the best performance with 22.86 than other treatments. Hence Probiotics i.e. Microguard has good performance in terms of growth rate and feed utilization than other probiotics.
Keywords: Mustard Oil Cake (MOC); Walking catfish; Probiotics; Microguard; Nicoli; Grozyme
Int. J. Grad. Res. Rev.Vol-4, Issue-4: 129-133
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