Relationship between Biofilm Formation and Antibiotic Susceptibility Pattern in Uropathogenic Escherichia coli
Rejina Shrestha*, Anisha Shrestha1, Binod Khadka1, Rosy K.C.1, Manju Shree Shakya(Hada)1, Anil Kumar Sah2
1Department of Microbiology, Tri-Chandra Multiple Campus, Ghantaghar Kathmandu, Nepal
2Annapurna Research Centre, Maitighar, Kathmandu, Nepal
Rejina Shrestha*, Anisha Shrestha1, Binod Khadka1, Rosy K.C.1, Manju Shree Shakya(Hada)1, Anil Kumar Sah2
1Department of Microbiology, Tri-Chandra Multiple Campus, Ghantaghar Kathmandu, Nepal
2Annapurna Research Centre, Maitighar, Kathmandu, Nepal
Int. J. Grad. Res. Rev.Vol-4, Issue-2: 44-53
Copyright (c) 2018 International Journal of Graduate Research and Review
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Abstract
Escherichia coli are one of the most common isolates from the urine sample. The ease of treatment of the UPEC mediated UTI is hindered by many factors developed within the pathogen-biofilm being one of the factor resulting in the resistance of pathogen against the prevalent antibiotics and emergence of MDR cases. Biofilm formation by E.coli is a pathogenic mechanism in which the organism covers itself by exopolysaccharide coat and the organism becomes resistant to antibiotics which are used to tackle the pathogen. The study was done to understand the relationship between biofilm formation and antibiotic susceptibility pattern in Uropathogenic E. coli. For this study, a total of 350 urine sample was analyzed and 48 UPEC isolates were isolated from suspected urinary tract infected patients at Annapurna Neuro Hospital, Kathmandu from April 2017 to September 2017. The isolates were characterized by biochemical tests and were subjected to AST which was done by modified Kirby- Bauer disk diffusion method. In-vitro biofilm production by these isolates was determined by Congo red agar method. The most effective antibiotic was found to be Imipenem (100%), followed by Nitrofurantoin (87.5%) and Amikacin (83.3%). Biofilm production was found in 60.4% of isolates. These isolates forming biofilm were found to be highly resistant to antibiotics. Biofilm production makes the organism to be more resistant to antibiotics and virulent as compared to non-biofilm producers.
Escherichia coli are one of the most common isolates from the urine sample. The ease of treatment of the UPEC mediated UTI is hindered by many factors developed within the pathogen-biofilm being one of the factor resulting in the resistance of pathogen against the prevalent antibiotics and emergence of MDR cases. Biofilm formation by E.coli is a pathogenic mechanism in which the organism covers itself by exopolysaccharide coat and the organism becomes resistant to antibiotics which are used to tackle the pathogen. The study was done to understand the relationship between biofilm formation and antibiotic susceptibility pattern in Uropathogenic E. coli. For this study, a total of 350 urine sample was analyzed and 48 UPEC isolates were isolated from suspected urinary tract infected patients at Annapurna Neuro Hospital, Kathmandu from April 2017 to September 2017. The isolates were characterized by biochemical tests and were subjected to AST which was done by modified Kirby- Bauer disk diffusion method. In-vitro biofilm production by these isolates was determined by Congo red agar method. The most effective antibiotic was found to be Imipenem (100%), followed by Nitrofurantoin (87.5%) and Amikacin (83.3%). Biofilm production was found in 60.4% of isolates. These isolates forming biofilm were found to be highly resistant to antibiotics. Biofilm production makes the organism to be more resistant to antibiotics and virulent as compared to non-biofilm producers.
