Sah_et_al.

Growth Pattern of Economically Important Fungi in Fruits Peel Waste Media
Shiv Nandan Sah*, Tara Ghimire¹, Surendra Prasad Yadav² and Pradeep Kumar Shah³
¹Department of Microbiology, Central Campus of Technology, Dharan, Tribhuvan University,Nepal
²Department of Microbiology, Mahendra Morang Adarsha Multiple Campus, Morang, Tribhuvan University, Nepal
³Department of Microbiology, Tri-Chandra Multiple Campus, Ghantaghar, Kathmandu,Tribhuvan University, Nepal

Abstract
Fruits peels, the unused outer covering part, are considered as fruits peel waste which can be simply used in different microbiological media preparing in various industries. This study was aimed to formulate growth media for some economically important fungi by using fruits peel waste materials such as Pineapple, Papaya, Green Banana, Yellow Banana, Orange and Pomegranate. Fresh Fruits were purchased from Local market of Dharan and obtained fruits peels were air dried and grinded separately into fine particles using mechanical blender and then sieved with 1mm sieve size. Fungi isolates (Aspergillus niger, Rhizopus stoloniferand Saccharomyces cerevisiae) were inoculated separately into the medium prepared in conical flask by using fruits peel powder (4 g in 100mL distilled water) and incubated at room temperature (25°C±1) for 5 days. Growth of Aspergillus niger was recorded in the medium containing Pineapple, Orange, Green Banana, Yellow Banana and Pomegranate except Papaya while Rhizopus stolonifer growth was observed in the medium containing Pineapple, Green Banana, Yellow Banana and Pomegranate except Papaya and Orange. However, the growth of Saccharomyces cerevisiae was recorded in the medium containing Pineapple, Green Banana, Orange and Papaya except Yellow banana and Pomegranate. This study showed that some economically important fungi can be successfully grown in some fruits peel waste media.
Keywords: Fruits peel waste; economically important fungi; Aspergillus niger; Rhizopus stolonifera; Saccharomyces cerevisiae